The working group began under the name of “Histology Laboratory” of the National Institute of Fisheries Research and Development (INIDEP). Our working group was renamed as “Reproductive Ecology of Marine Organisms” (ECOR) in 2015. The activities of ECOR group are currently enclosed in different research programs of INIDEP. We also participate in projects founded by other national and international organizations. Since the early 1990s, the tasks of this lab have been focused on the investigation of those aspects of importance for the evaluation of fishery resources. We use histology as the main research tool, and apply the obtained information in a context of population analysis. We have worked mainly on reproduction, since it is the biological process of major relevance in the renewal of populations. In this sense, during these years of research we have been able to determine aspects such as the reproductive dynamic, pattern of spawning, ripening process, reproductive potential and spatial and temporal delimitation of spawning process in more than 30 species of fish and marine invertebrates. Other related topics have also been studied, such as the pattern of energy distribution of reproductive females according to the annual cycle, and nutritional condition during the different stages of the life history of organisms. Currently, we are also conducting studies on reproductive physiology by the determination of hormones concentration, in particular in elasmobranch fish. In all cases, biological information is analyzed in the context of the physical environment, which allowed describing reproductive strategies of the species, to define variations in the spawning areas, the recruitment mechanisms and the processes of mortality associated with the oceanographic conditions.
In terms of the training of human resources, during these years the research group has trained numerous undergraduate and PhD students from the National University of Buenos Aires and National University of Mar del Plata. We also directed research scholarships founded by INIDEP and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Moreover we have obtained grants from the “Fondo para la Investigación Científica y Tecnológica” (FONCyT) and CONICET, and participated in the evaluation of research projects, scholarships, competitions, career income of CONICET, promotions, peer reviewed international publications and participated in the organization of international scientific meetings.
General objective

Contribute to the knowledge of the reproductive biology of marine organisms, in order to evaluate the effects of fishing and environmental changes on the reproductive potential of the populations and their possible relation with the variability of the recruitment.

Specific objectives

To describe the maturation process and to determine the pattern of spawning of marine species from the macroscopic, histological, biochemical and physiological analysis of spawners.
To determine the spawning areas and the reproductive cycle of the species.
To characterize the reproductive areas based on oceanographic analysis.
To estimate the reproductive potential of the fish stocks.
To estimate length and age at maturity of the fish stocks.
To evaluate the egg quality from the proximal composition of spawners.
To analyze the larval condition using histological techniques and biochemical methods (RNA/DNA).
To analyze the effects of the fishery exploitation and environmental changes on the reproductive potential and recruitment of stocks.
To compare the reproductive strategies adopted by different marine species according to their life histories and physical conditions in the spawning areas.

– Reproductive ecology in marine organisms.
– Histology applied to the description and quantification of reproductive cells and larval tissues.
– Biochemical composition of tissues for determination of condition in fish.
– Reproductive physiology of fish.
– Nucleic acids determination as condition index in fish larvae.
– Population dynamic in exploited fish stocks.

Director
Dr. Gustavo Javier Macchi
Researcher CONICET
Novillo, M., Moreira, E., Macchi, G., & Barrera-Oro, E. (en prensa). Reproductive effort in Chaenocephalus aceratus validated by gonadal histology: inshore sites serve as spawning grounds for some notothenioids. Polar Biology. https://doi.org/10.1007/s00300-019-02571-8
Marrari, M., Macchi, G. J., Santos, B., & Leonarduzzi, E. (2019). Influence of environmental conditions on the reproductive success and recruitment of the Argentine hake Merluccius hubbsi (southwestern Atlantic Ocean). Fisheries Oceanography, 28(1), 66–81. https://doi.org/10.1111/fog.12387
Novillo, M., Moreira, E., Macchi, G., & Barrera-Oro, E. (2018). Reproductive biology in the Antarctic bathydraconid dragonfish Parachaenichthys charcoti. Polar Biology, 41(11), 2239–2248. https://doi.org/10.1007/s00300-018-2359-5
Wehitt, A., Colonello, J. H., Macchi, G. J., Galíndez, E. J., Wehitt, A., Colonello, J. H., … Galíndez, E. J. (2018). Reproductive biology of the eyespot skate Atlantoraja cyclophora (Elasmobranchii: Arhynchobatidae) an endemic species of the Southwestern Atlantic Ocean (34oS - 42oS). Neotropical Ichthyology, 16(2), e170098. https://doi.org/10.1590/1982-0224-20170098
Macchi, G. J., Rodrigues, K., Leonarduzzi, E., & Diaz, M. V. (2018). Is the spawning frequency of Argentine hake, Merluccius hubbsi, affected by maternal attributes or physical variables? Fisheries Research, 204, 147–155. https://doi.org/10.1016/j.fishres.2018.02.011
Militelli, M. I., López, S., Rodrigues, K. A., García, S., & Macchi, G. J. (2017). Reproductive potential of Pagrus pagrus (Perciformes: Sparidae) in coastal waters of Buenos Aires Province (Argentina) and Uruguay (34o-39oS). Neotropical Ichthyology, 15(2), e160127. https://doi.org/10.1590/1982-0224-20160127
Fernández, M., Iorio, M. I., Hernández, D., & Macchi, G. (2012). Studies on the reproductive dynamics of Pleoticus muelleri (Bate, 1888) (Crustacea, Decapoda, Solenoceridae) of Patagonia, Argentina. Latin American Journal of Aquatic Research, 40(4), 858–871. Retrieved from http://www.redalyc.org/resumen.oa?id=175025197018
Ehrlich, M., Macchi, G., Madirolas, A., & Machinandiarena, L. (2013). Vertical distribution of hake Merluccius hubbsi in spawning aggregations in North Patagonian waters of the Southwest Atlantic. Fisheries Research, 138, 89–98. https://doi.org/10.1016/j.fishres.2012.07.007
Colonello, J. H., Christiansen, H. E., Cousseau, M. B., & Macchi, G. J. (2013). Uterine dynamics of the southern eagle ray Myliobatis goodei (Chondrichthyes: Myliobatidae) from the southwest Atlantic Ocean. Italian Journal of Zoology, 80(2), 187–194. https://doi.org/10.1080/11250003.2012.742146
Militelli, M. I., Macchi, G. J., & Rodrigues, K. A. (2013). Comparative reproductive biology of Sciaenidae family species in the Río de la Plata and Buenos Aires Coastal Zone, Argentina. Journal of the Marine Biological Association of the United Kingdom, 93(2), 413–423. https://doi.org/10.1017/S0025315412001488
Militelli, M. I., Rodrigues, K. A., Cortés, F., & Macchi, G. J. (2013). Influence of environmental factors on the spawning of sciaenids in the Buenos Aires Coastal Zone, Argentina. Ciencias Marinas, 39(1), 55–68. https://doi.org/10.7773/cm.v39i1.2176
Rodrigues, K. A., Macchi, G. J., Massa, A., & Militelli, M. I. (2013). Seasonal analysis of condition, biochemical and bioenergetic indices of females of Brazilian flathead, Percophis brasiliensis. Neotropical Ichthyology, 11(1), 153–162. https://doi.org/10.1590/S1679-62252013000100018
Macchi, G. J., Leonarduzzi, E., Diaz, M. V., Renzi, M., & Rodrigues, K. (2013). Maternal effects on fecundity and egg quality of the Patagonian stock of Argentine Hake (Merluccius hubbsi). Fishery Bulletin, 111(4), 325–336. https://doi.org/Macchi, Gustavo J.  and Leonarduzzi, Ezequiel and Diaz, Marina V. and Renzi, Marta and Rodrigues, Karina          (2013) Maternal effects on fecundity and egg quality of the Patagonian stock of Argentine Hake (Merluccius hubbsi).  Fishery Bulletin,  111(4),  pp. 325-336. 10.7755/FB.111.4.3  <http://dx.doi.org/10.7755/FB.111.4.3 >
Diaz, M. V., Arano, M. F., Pájaro, M., Aristizábal, E. O., & Macchi, G. J. (2013). The use of morphological and histological features as nutritional condition indices of Pagrus pagrus larvae. Neotropical Ichthyology, 11(3), 649–660. https://doi.org/10.1590/S1679-62252013000300018
Diaz, M. V., Olivar, M. P., & Macchi, G. J. (2014). Larval condition of Merluccius hubbsi (Marini, 1933) in the northern Patagonian spawning ground. Fisheries Research, 160, 60–68. https://doi.org/10.1016/j.fishres.2013.11.009
Leonarduzzi, E., Rodrigues, K. A., & Macchi, G. J. (2014). Proximate composition and energy density in relation to Argentine hake females (Merluccius hubbsi) morphometrics and condition indices. Fisheries Research, 160, 33–40. https://doi.org/10.1016/j.fishres.2014.04.017
Macchi, G. J., Saborido-Rey, F., Murua, H., Claramunt, G., & De Tarso Chaves, P. (2014). Advances in fisheries research in Ibero-America. Fisheries Research, 160, 1–7. https://doi.org/10.1016/j.fishres.2014.06.002
Fernández, M., Hernández, D., & Macchi, G. (2014). Variación espacio-temporal del estado reproductivo de los machos de langostino Pleoticus muelleri (Spence Bate, 1888) (Crustacea, Decapoda, Solenoceridae) en el litoral patagónico, Argentina. Revista de Investigación y Desarrollo Pesquero, 24, 37–57. Retrieved from http://www.oceandocs.org:80/handle/1834/6774
Rodrigues, K. A., Macchi, G. J., & Militelli, M. I. (2015). Comparative study of spawning pattern and reproductive potential of the Northern and Southern stocks of Argentine hake (Merluccius hubbsi). Journal of Sea Research, 102, 22–32. https://doi.org/10.1016/j.seares.2015.04.002
Militelli, M. I., Macchi, G. J., & Rodrigues, K. A. (2015). Maturity and fecundity of Champsocephalus gunnari, Chaenocephalus aceratus and Pseudochaenichthys georgianus in South Georgia and Shag Rocks Islands. Polar Science, 9(2), 258–266. https://doi.org/10.1016/j.polar.2015.03.004
Villanueva-Gomila, G. L., Macchi, G. J., Ehrlich, M. D., Irigoyen, A. J., & Venerus, L. A. (2015). The reproductive biology of Pinguipes brasilianus Cuvier, 1829 (Osteichthyes: Pinguipedidae) in temperate rocky reefs of Argentina. Neotropical Ichthyology, 13(4), 733–744. https://doi.org/10.1590/1982-0224-20150053
Elisio, M., Colonello, J. H., Cortés, F., Jaureguizar, A. J., Somoza, G. M., & Macchi, G. J. (2017). Aggregations and reproductive events of the narrownose smooth-hound shark (Mustelus schmitti) in relation to temperature and depth in coastal waters of the south-western Atlantic Ocean (38–42°S). Marine and Freshwater Research, 68(4), 732–742. https://doi.org/10.1071/MF15253
Irusta, G., Macchi, G. J., Louge, E., Rodrigues, K. A., D’Atri, L. L., Villarino, M. F., … Simonazzi, M. (2016). Biology and fishery of the Argentine hake (Merluccius hubbsi). Revista de Investigación y Desarrollo Pesquero, 28, 9–36. Retrieved from http://marabierto.inidep.edu.ar/handle/inidep/1665
Macchi, G. J., Rodrigues, K., Diaz, M. V., & Militelli, M. (2017). Effects of skipped spawning on the reproductive potential of Argentine hake (Merluccius hubbsi). Fishery Bulletin, 115(3), 396–407. https://doi.org/10.7755/FB.115.3.9
Díaz, A., Freijo, R. O., García, A. M., Portiansky, E. L., Barbeito, C. G., & Macchi, G. J. (2012). Lectin histochemical aspects of the ovarian lamellae epithelium of Genypterus blacodes (Schneider, 1801). Pesquisa Veterinária Brasileira, 32(6), 547–552. Retrieved from http://www.scielo.br/scielo.php?pid=S0100-736X2012000600013&script=sci_arttext&tlng=pt
Cohen, S., Petcoff, G., Freijo, R. O., Portiansky, E. L., Barbeito, C. G., Macchi, G. J., & Díaz, A. (2015). Histochemical characterization of oocytes in the pink cuskeel (Genypterus blacodes). Zoological Science, 32(4), 383–388. Retrieved from https://www.bioone.org/doi/full/10.2108/zs140235
Cohen, S., Freijo, R. O., Petcoff, G. M., Portiansky, E. L., Barbeito, C. G., Macchi, G. J., & Díaz, A. (2016). Glicoconjugados del ovario de Genypterus blacodes y su importancia en la biología reproductiva. Ciencias Morfológicas, 18(1), 1–9. Retrieved from http://sedici.unlp.edu.ar/handle/10915/55444
Macchi, G. J., Diaz, M. V., Leonarduzzi, E., Militelli, M. I., & Rodrigues, K. (2016). Skipped spawning in the Patagonian stock of Argentine hake (Merluccius hubbsi). Fishery Bulletin, 114(4).
Researchers
Dra. María Inés Militelli
Researcher CONICET
Militelli, M. I., López, S., Rodrigues, K. A., García, S., & Macchi, G. J. (2017). Reproductive potential of Pagrus pagrus (Perciformes: Sparidae) in coastal waters of Buenos Aires Province (Argentina) and Uruguay (34o-39oS). Neotropical Ichthyology, 15(2), e160127. https://doi.org/10.1590/1982-0224-20160127
Militelli, M. I., Macchi, G. J., & Rodrigues, K. A. (2013). Comparative reproductive biology of Sciaenidae family species in the Río de la Plata and Buenos Aires Coastal Zone, Argentina. Journal of the Marine Biological Association of the United Kingdom, 93(2), 413–423. https://doi.org/10.1017/S0025315412001488
Militelli, M. I., Rodrigues, K. A., Cortés, F., & Macchi, G. J. (2013). Influence of environmental factors on the spawning of sciaenids in the Buenos Aires Coastal Zone, Argentina. Ciencias Marinas, 39(1), 55–68. https://doi.org/10.7773/cm.v39i1.2176
Rodrigues, K. A., Macchi, G. J., Massa, A., & Militelli, M. I. (2013). Seasonal analysis of condition, biochemical and bioenergetic indices of females of Brazilian flathead, Percophis brasiliensis. Neotropical Ichthyology, 11(1), 153–162. https://doi.org/10.1590/S1679-62252013000100018
Rodrigues, K. A., Macchi, G. J., & Militelli, M. I. (2015). Comparative study of spawning pattern and reproductive potential of the Northern and Southern stocks of Argentine hake (Merluccius hubbsi). Journal of Sea Research, 102, 22–32. https://doi.org/10.1016/j.seares.2015.04.002
Militelli, M. I., Macchi, G. J., & Rodrigues, K. A. (2015). Maturity and fecundity of Champsocephalus gunnari, Chaenocephalus aceratus and Pseudochaenichthys georgianus in South Georgia and Shag Rocks Islands. Polar Science, 9(2), 258–266. https://doi.org/10.1016/j.polar.2015.03.004
Macchi, G. J., Rodrigues, K., Diaz, M. V., & Militelli, M. (2017). Effects of skipped spawning on the reproductive potential of Argentine hake (Merluccius hubbsi). Fishery Bulletin, 115(3), 396–407. https://doi.org/10.7755/FB.115.3.9
Macchi, G. J., Diaz, M. V., Leonarduzzi, E., Militelli, M. I., & Rodrigues, K. (2016). Skipped spawning in the Patagonian stock of Argentine hake (Merluccius hubbsi). Fishery Bulletin, 114(4).
Acha, E. M., Rodrigues, K. A., Militelli, M. I., Orduna, M., & Braverman, M. S. (2012). Caracterización de la zona de “El Rincón” (provincia de Buenos Aires) como área de reproducción de peces costeros. Revista de Investigación y Desarrollo Pesquero, 21, 31–43. Retrieved from http://www.oceandocs.org/handle/1834/4910
Dra. Marina Vera Diaz
Researcher CONICET
Dra. Karina Andrea Rodrigues
Researcher CONICET
Macchi, G. J., Rodrigues, K., Leonarduzzi, E., & Diaz, M. V. (2018). Is the spawning frequency of Argentine hake, Merluccius hubbsi, affected by maternal attributes or physical variables? Fisheries Research, 204, 147–155. https://doi.org/10.1016/j.fishres.2018.02.011
Militelli, M. I., López, S., Rodrigues, K. A., García, S., & Macchi, G. J. (2017). Reproductive potential of Pagrus pagrus (Perciformes: Sparidae) in coastal waters of Buenos Aires Province (Argentina) and Uruguay (34o-39oS). Neotropical Ichthyology, 15(2), e160127. https://doi.org/10.1590/1982-0224-20160127
Militelli, M. I., Macchi, G. J., & Rodrigues, K. A. (2013). Comparative reproductive biology of Sciaenidae family species in the Río de la Plata and Buenos Aires Coastal Zone, Argentina. Journal of the Marine Biological Association of the United Kingdom, 93(2), 413–423. https://doi.org/10.1017/S0025315412001488
Militelli, M. I., Rodrigues, K. A., Cortés, F., & Macchi, G. J. (2013). Influence of environmental factors on the spawning of sciaenids in the Buenos Aires Coastal Zone, Argentina. Ciencias Marinas, 39(1), 55–68. https://doi.org/10.7773/cm.v39i1.2176
Rodrigues, K. A., Macchi, G. J., Massa, A., & Militelli, M. I. (2013). Seasonal analysis of condition, biochemical and bioenergetic indices of females of Brazilian flathead, Percophis brasiliensis. Neotropical Ichthyology, 11(1), 153–162. https://doi.org/10.1590/S1679-62252013000100018
Macchi, G. J., Leonarduzzi, E., Diaz, M. V., Renzi, M., & Rodrigues, K. (2013). Maternal effects on fecundity and egg quality of the Patagonian stock of Argentine Hake (Merluccius hubbsi). Fishery Bulletin, 111(4), 325–336. https://doi.org/Macchi, Gustavo J.  and Leonarduzzi, Ezequiel and Diaz, Marina V. and Renzi, Marta and Rodrigues, Karina          (2013) Maternal effects on fecundity and egg quality of the Patagonian stock of Argentine Hake (Merluccius hubbsi).  Fishery Bulletin,  111(4),  pp. 325-336. 10.7755/FB.111.4.3  <http://dx.doi.org/10.7755/FB.111.4.3 >
Leonarduzzi, E., Rodrigues, K. A., & Macchi, G. J. (2014). Proximate composition and energy density in relation to Argentine hake females (Merluccius hubbsi) morphometrics and condition indices. Fisheries Research, 160, 33–40. https://doi.org/10.1016/j.fishres.2014.04.017
Rodrigues, K. A., Macchi, G. J., & Militelli, M. I. (2015). Comparative study of spawning pattern and reproductive potential of the Northern and Southern stocks of Argentine hake (Merluccius hubbsi). Journal of Sea Research, 102, 22–32. https://doi.org/10.1016/j.seares.2015.04.002
Militelli, M. I., Macchi, G. J., & Rodrigues, K. A. (2015). Maturity and fecundity of Champsocephalus gunnari, Chaenocephalus aceratus and Pseudochaenichthys georgianus in South Georgia and Shag Rocks Islands. Polar Science, 9(2), 258–266. https://doi.org/10.1016/j.polar.2015.03.004
Irusta, G., Macchi, G. J., Louge, E., Rodrigues, K. A., D’Atri, L. L., Villarino, M. F., … Simonazzi, M. (2016). Biology and fishery of the Argentine hake (Merluccius hubbsi). Revista de Investigación y Desarrollo Pesquero, 28, 9–36. Retrieved from http://marabierto.inidep.edu.ar/handle/inidep/1665
Macchi, G. J., Rodrigues, K., Diaz, M. V., & Militelli, M. (2017). Effects of skipped spawning on the reproductive potential of Argentine hake (Merluccius hubbsi). Fishery Bulletin, 115(3), 396–407. https://doi.org/10.7755/FB.115.3.9
Macchi, G. J., Diaz, M. V., Leonarduzzi, E., Militelli, M. I., & Rodrigues, K. (2016). Skipped spawning in the Patagonian stock of Argentine hake (Merluccius hubbsi). Fishery Bulletin, 114(4).
Acha, E. M., Rodrigues, K. A., Militelli, M. I., Orduna, M., & Braverman, M. S. (2012). Caracterización de la zona de “El Rincón” (provincia de Buenos Aires) como área de reproducción de peces costeros. Revista de Investigación y Desarrollo Pesquero, 21, 31–43. Retrieved from http://www.oceandocs.org/handle/1834/4910