The general objective of our research group is to carry out studies on phenotypic flexibility at the biochemical and physiological level in key organisms from coastal environments. Within the general objective, we carried out integrative and comparative studies on digestive and metabolic biochemical physiology and on mechanisms of adjustment at the biochemical-physiological level in relation to key environmental variables as well as  on the pathways and regulatory mechanisms involved.

Integrative and comparative studies on enzymatic activities involved in adjustments to changes in key environmental variables and on pathways and regulatory mechanisms involved

Studies of characterization and of modulation by different pathways of key enzymes in the digestive tract and of energy reserves.

Director    
Dra. Lopez Mañanes Alejandra Antonia
Researcher CONICET
Professor
Méndez, E., Michiels, M. S., & López-Mañanes, A. A. (2021). The influence of habitat on metabolic and digestive parameters in an intertidal crab from a SW Atlantic coastal lagoon. Belgian Journal of Zoology, 151, 81–98. https://doi.org/10.26496/bjz.2021.87
Albanesi, C., González‐Castro, M., & López Mañanes, A. (2021). Understanding the early ontogenetic stages of Mugil liza (Mugilidae): Morphological traits and digestive/metabolic profile of pre-juveniles after recruitment. Journal of Fish Biology, 98(3), 643–654. https://doi.org/https://doi.org/10.1111/jfb.14605
Michiels, M. S., Daleo, G. R., & López Mañanes, A. A. (2020). Differential modulation after feeding in different salinities and response to abscisic acid (ABA) and extracellular Ca2+ of aminopeptidase N (APN) activity in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata. Canadian Journal of Zoology, 98(4), 262–268. https://doi.org/10.1139/cjz-2019-0163
Lopez Mañanes, A. A., Asaro, A., Méndez, E., Michiels, M. S., & Pinoni, S. A. (2020). Digestive flexibility in Neohelice granulata from the Mar Chiquita coastal lagoon: Characterization and modulation of key enzymes in hepatopancreas. In E. M. Rodriguez & T. A. Luppi (Eds.), Neohelice granulata, a Model Species for Studies on Crustaceans, Volume II. Cambridge Scholars Publishing. https://ri.conicet.gov.ar/handle/11336/128920
Asaro, A., Paggi, R. A., del Valle, J. C., & López Mañanes, A. A. (2018). Glucose homeostasis in the euryhaline crab Cytograpsus angulatus: Effects of the salinity in the amylase, maltase and sucrase activities in the hepatopancreas and in the carbohydrate reserves in different tissues. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 216, 39–47. https://doi.org/10.1016/j.cbpb.2017.11.012
Pinoni, S. A., Jerez-Cepa, I., López Mañanes, A. A., & Romero, J. M. M. (2018). The euryhaline crab Uca tangeri showed metabolic differences to sex and environmental salinity. Journal of the Marine Biological Association of the United Kingdom, 98(6), 1465–1475. https://doi.org/10.1017/S0025315417000601
Méndez, E., Caruso Neves, C., & López Mañanes, A. (2018). Two sodium pumps in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata: biochemical characteristics and differential modulation after feeding. Canadian Journal of Zoology, 96(6), 576–584. https://doi.org/10.1139/cjz-2017-0128
Asaro, A., Martos-Sitcha, J. A., Martínez-Rodríguez, G., Mancera, J. M., & López Mañanes, A. (2018). In silico analysis and effects of environmental salinity in the expression and activity of digestive α-amylase and trypsins from the euryhaline crab Neohelice granulata. Canadian Journal of Zoology, 96(2), 127–139. https://doi.org/10.1139/cjz-2016-0324
Michiels, M. S., del Valle, J. C., & López Mañanes, A. A. (2017). Trypsin and N-aminopeptidase (APN) activities in the hepatopancreas of an intertidal euryhaline crab: Biochemical characteristics and differential modulation by histamine and salinity. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 204, 228–235.
Pinoni, S. A., & López Mañanes, A. A. (2016). Digestive flexibility in the euryhaline crab Cyrtograpsus angulatus (Decapoda, Brachyura, Varunidae) from Mar Chiquita coastal lagoon (Buenos Aires, Argentina): responses to salinity of key enzymes in hepatopancreas. Pan-American Journal of Aquatic Sciences, 11(4), 345–355. http://www.panamjas.org/pdf_artigos/PANAMJAS_11(4)_345-355.pdf
Del Valle, J. C., Michiels, M. S., Radonic, M., López, A., & López Mañanes, A. A. (2016). Digestive and metabolic profile at the biochemical level of juvenile flounder Paralichthys orbignyanus (Valenciennes, 1839) (Pleuronectiformes: Paralichthyidae). Pan-American Journal of Aquatic Sciences, 11(4), 309–323. http://www.panamjas.org/pdf_artigos/PANAMJAS_11(4)_309-323.pdf
Del Valle, J. C., Acuña, F. H., & López Mañanes, A. A. (2015). Digestive flexibility in response to environmental salinity and temperature in the non-symbiotic sea anemone Bunodosoma zamponii. Hydrobiologia, 759(1), 189–199.
Pinoni, S. A., Méndez, E., & López Mañanes, A. A. (2015). Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: alkaline phosphatase activity sensitive to salinity in the hepatopancreas. Journal of the Marine Biological Association of the United Kingdom, 95(06), 1133–1140.
Michiels, M. S., del Valle, J. C., & López Mañanes, A. A. (2015). Lipase activity sensitive to dopamine, glucagon and cyclic AMP in the hepatopancreas of the euryhaline burrowing crab Neohelice granulata (Dana, 1851)(Decapoda, Grapsidae). Crustaceana, 88(1), 51–65. http://booksandjournals.brillonline.com/content/journals/10.1163/15685403-00003399
Michiels, M. S., Del Valle, J. C., & López Mañanes, A. A. (2015). Biochemical characteristics and modulation by external and internal factors of aminopeptidase-N activity in the hepatopancreas of a euryhaline burrowing crab. Journal of Comparative Physiology B, 185(5), 501–510. http://link.springer.com/article/10.1007/s00360-015-0899-3
Pinoni, S. A., Michiels, M. S., & López Mañanes, A. A. (2013). Phenotypic flexibility in response to environmental salinity in the euryhaline crab Neohelice granulata from the mudflat and the saltmarsh of a SW coastal lagoon. Marine Biology, 160(10), 2647–2661.
Michiels, M. S., del Valle, J. C., & López Mañanes, A. A. (2013). Effect of environmental salinity and dopamine injections on key digestive enzymes in hepatopancreas of the euryhaline crab Cyrtograpsus angulatus (Decapoda: Brachyura: Varunidae. Scientia Marina, 77(1), 129–136. http://ri.conicet.gov.ar/handle/11336/2234
Researchers    
Dr. González Castro Mariano
Researcher CONICET
Albanesi, C., González‐Castro, M., & López Mañanes, A. (2021). Understanding the early ontogenetic stages of Mugil liza (Mugilidae): Morphological traits and digestive/metabolic profile of pre-juveniles after recruitment. Journal of Fish Biology, 98(3), 643–654. https://doi.org/https://doi.org/10.1111/jfb.14605
Hughes, L. C., Cardoso, Y. P., Sommer, J. A., Cifuentes, R., Cuello, M., Somoza, G. M., González‐Castro, M., Malabarba, L. R., Cussac, V., Habit, E. M., Betancur‐R, R., & Ortí, G. (2020). Biogeography, habitat transitions and hybridization in a radiation of South American silverside fishes revealed by mitochondrial and genomic RAD data. Molecular Ecology, 29(4), 738–751. https://doi.org/10.1111/mec.15350
Bianchi, S. J., & González-Castro, M. (2020). Reproductive biology of Parona signata (Actinopterygii: Carangidae), a valuable economic resource, in the coastal area of Mar del Plata, Buenos Aires, Argentina. Neotropical Ichthyology, 18(3), e190133. https://doi.org/10.1590/1982-0224-2019-0133
Colautti, D., Miranda, L., Gonzalez‐Castro, M., Villanova, G. V., Strüssmann, C. A., Mancin, M., Maiztegui, T., Berasain, G., Hattori, R. S., Grosman, F., Sanzano, P., Lozano, I., Vegh, S. L., Salinas, V., Ponti, O. D., Fresno, P. del, Minotti, P., Yamamoto, Y., & Baigún, C. (2020). Evidence of a landlocked reproducing population of the marine pejerrey Odontesthes argentinensis (Actinopterygii; Atherinopsidae). Journal of Fish Biology, 96(1), 202–216. https://doi.org/10.1111/jfb.14207
Deli Antoni, M. Y., Delpiani, S. M., González-Castro, M., Blasina, G. E., Spath, M. C., Depiani, G. E., Ashikaga, F. Y., Cruz, V. P., Oliveira, C., & Díaz de Astarloa, J. M. (2019). Comparative populational study of Lepidonotothen larseni and L. nudifrons (Teleostei: Nototheniidae) from the Antarctic Peninsula and the South Shetland Islands, Antarctica. Polar Biology, 42(8), 1537–1547. https://doi.org/10.1007/s00300-019-02540-1
González-Castro, M., Rosso, J. J., Delpiani, S. M., Mabragaña, E., & Díaz de Astarloa, J. M. (2019). Inferring boundaries among fish species of the new world silversides (Atherinopsidae; genus Odontesthes): new evidences of incipient speciation between marine and brackish populations of Odontesthes argentinensis. Genetica, 147, 217–229. https://doi.org/10.1007/s10709-019-00066-2
Castellini, D. L., Brown, D., Lajud, N. A., Díaz de Astarloa, J. M., & González-Castro, M. (2019). Juveniles recruitment and daily growth of the southern stock of Mugil liza (Actinopterygii; Fam. Mugilidae): new evidence for the current life-history model. Journal of the Marine Biological Association of the United Kingdom, 99(1), 215–221. https://doi.org/10.1017/S0025315417001904
Cardoso, Y. P., Rosso, J. J., Mabragaña, E., González-Castro, M., Delpiani, M., Avigliano, E., Bogan, S., Covain, R., Schenone, N. F., & Díaz de Astarloa, J. M. (2018). A continental-wide molecular approach unraveling mtDNA diversity and geographic distribution of the Neotropical genus Hoplias. PLOS ONE, 13(8), e0202024. https://doi.org/10.1371/journal.pone.0202024
Castellini, D. L., González-Castro, M., Lopez-Cazorla, A., & Díaz de Astarloa, J. M. (2018). Abnormalities in flatfishes of the south-west Atlantic Ocean. Journal of Fish Biology, 92(4), 1225–1234. https://doi.org/10.1111/jfb.13578
Díaz de Astarloa, J. M., Munroe, T. A., Béarez, P., Gonzalez-Castro, M., Castellini, D. L., Astarloa, J. M. D. de, Munroe, T. A., Béarez, P., Gonzalez-Castro, M., & Castellini, D. L. (2018). External morphology, postcranial and appendicular osteology of three southwestern Atlantic flatfishes ( Paralichthys , Paralichthyidae), and comparisons with other congeneric species. Neotropical Ichthyology, 16(2), e170134. https://doi.org/10.1590/1982-0224-20170164
Callicó Fortunato, R., González-Castro, M., Reguera Galán, A., García Alonso, I., Kunert, C., Benedito Durà, V., & Volpedo, A. (2017). Identification of potential fish stocks and lifetime movement patterns of Mugil liza Valenciennes 1836 in the Southwestern Atlantic Ocean. Fisheries Research, 193, 164–172. https://doi.org/10.1016/j.fishres.2017.04.005
Callicó Fortunato, R., Benedito Durà, V., González-Castro, M., & Volpedo, A. (2017). Morphological and morphometric changes of sagittae otoliths related to fish growth in three Mugilidae species. Journal of Applied Ichthyology, 33(6), 1137–1145. https://doi.org/10.1111/jai.13479
Hughes, L. C., Somoza, G. M., Nguyen, B. N., Bernot, J. P., González-Castro, M., Díaz de Astarloa, J. M., & Ortí, G. (2017). Transcriptomic differentiation underlying marine-to-freshwater transitions in the South American silversides Odontesthes argentinensis and O. bonariensis (Atheriniformes). Ecology and Evolution, 7(14), 5258–5268. https://doi.org/10.1002/ece3.3133
Rosso, J. J., Mabragaña, E., González-Castro, M., Delpiani, M., Avigliano, E., Schenone, N., & Díaz de Astarloa, J. M. (2016). A new species of the Hoplias malabaricus species complex (Characiformes: Erythrinidae) from the La Plata River basin. Cybium, 40(3), 199–208.
Mabragaña, E., Delpiani, M., Rosso, J. J., González-Castro, M., Deli Antoni, M., Hanner, R., & Díaz de Astarloa, J. M. (2016). Barcoding antarctic fishes: species discrimination and contribution to elucidate ontogenetic changes in nototheniidae. In DNA Barcoding in Marine Perspectives (pp. 213–242). Springer. http://link.springer.com/chapter/10.1007/978-3-319-41840-7_14
Lajud, N. A., Díaz de Astarloa, J. M., & González-Castro, M. (2016). Reproduction of Brevoortia aurea (Spix & Agassiz, 1829) (Actinopterygii: Clupeidae) in the Mar Chiquita Coastal Lagoon, Buenos Aires, Argentina. Neotropical Ichthyology, 14(1). http://www.scielo.br/scielo.php?pid=S1679-62252016000100222&script=sci_arttext
Devincenti, C. V., Longo, M. V., González-Castro, M., & Díaz, A. (2015). Morphological and histochemical characterization of the pectoral fin muscle of the stripped weakfish, Cynoscion guatucupa. Acta Zoologica, 96(2), 199–208. https://doi.org/10.1111/azo.12067
González-Castro, M., Rosso, J. J., Lajud, N. A., Castellini, D. L., & Díaz de Astarloa, J. M. (2015). Occurrence of Cyprinus carpio (Linnaeus, 1758) in a World Biosphere Reserve, the Mar Chiquita Coastal Lagoon (Argentina). Journal of Applied Ichthyology, 31(4), 749–751. https://doi.org/10.1111/jai.12780
Deli Antoni, M. Y., Delpiani, M., Stewart, A. L., González-Castro, M., & Díaz de Astarloa, J. M. (2015). Merluccius tasmanicus Matallanas & Lloris 2006 is a junior synonym of M. australis (Hutton 1872)(Gadiformes: Merluciidae) based on morphological and molecular data. Zootaxa, 3956(1), 029–055.
Deli Antoni, M. Y., González-Castro, M., & Díaz de Astarloa, J. M. (2015). New tools (DNA barcoding), old hypothesis: the case of the taxonomic identity of the Argentine hakes (Actinopterygii: Merluccius). Journal of Fish Biology, 87(3), 783–793. http://onlinelibrary.wiley.com/doi/10.1111/jfb.12745/full
González-Castro, M., & Minos, G. (2015). Sexuality and Reproduction of Mugilidae. In D. Crosetti & S. Blaber (Eds.), Biology, Ecology and Culture of Grey Mullets (Mugilidae) (pp. 227–263). CRC Press.
López, S., Mabragaña, E., Díaz de Astarloa, J. M., & González-Castro, M. (2015). Reproductive studies of Anchoa marinii Hildebrand, 1943 (Actinopterygii: Engraulidae) in the nearby-coastal area of Mar Chiquita coastal lagoon, Buenos Aires, Argentina. Neotropical Ichthyology, 13(1), 221–228.
González-Castro, M., Ghasemzadeh, J., Crosetti, D., & Blaber, S. (2015). Morphology and morphometry based taxonomy of Mugilidae. In Biology, Ecology and Culture of Grey Mullets (Mugilidae) (pp. 1–21). CRC Press.
Orlando, P., González-Castro, M., & Mabragaña, E. (2015). New insights to discriminate between Sympterygia acuta Garman 1877 and Sympterygia bonapartii Müller & Henle, 1841 (Rajidae) of the Southwest Atlantic Ocean: on the use of geometric morphometrics and spinulation patterns. Journal of Applied Ichthyology, 31(2), 381–389. http://onlinelibrary.wiley.com/doi/10.1111/jai.12674/full
Tano de la Hoz, M. F., García, A. M., González-Castro, M., & Díaz, A. (2014). Histochemical and scanning electron microscopic approaches to gills in juveniles of Odontesthes argentinensis (Actinopterygii, Atherinopsidae). International Journal of Aquatic Science, 5, 154–166.
López, S., Corbo, M., Castellini, D., Lajud, N., & González-Castro, M. (2014). First record of Porichthys porosissimus Cuvier, 1829 (Actinopterygii: Batrachoidiformes) and Macrodon atricauda Günther, 1880 (Actinopterygii: Perciformes) in Mar Chiquita coastal lagoon, Buenos Aires, Argentina. Check List, 10(4), 947–949.
Mai, A. C., Mino, C. I., Marins, L. F., Monteiro-Neto, C., Miranda, L., Schwingel, P. R., Lemos, V. M., González-Castro, M., Castello, J. P., & Vieira, J. P. (2014). Microsatellite variation and genetic structuring in Mugil liza (Teleostei: Mugilidae) populations from Argentina and Brazil. Estuarine, Coastal and Shelf Science, 149, 80–86. http://www.sciencedirect.com/science/article/pii/S027277141400198X
González-Castro, M., Delpiani, M., Bruno, D. O., & Díaz de Astarloa, J. M. (2013). Short communication First occurrence of the Patagonian blennie, Eleginops maclovinus (Cuvier, 1830) and the silverside Odontesthes smitti (Lahille, 1929), in a temperate south-western Atlantic coastal lagoon. Journal of Applied Ichthyology, 29, 940–942. https://www.researchgate.net/profile/Mariano_Gonzalez-Castro/publication/236212418_First_occurrence_of_the_Patagonian_blennie_Eleginops_maclovinus_(Cuvier_1830)_and_the_silverside_Odontesthes_smitti_(Lahille_1929)_in_a_temperate_south-western_Atlantic_coastal_lagoon/links/558302bd08ae8bf4ba6f86cd.pdf
Mabragaña, E., Delpiani, M., Blasina, G. E., González-Castro, M., Rosso, J. J., & Díaz de Astarloa, J. M. (2012). Sardinella aurita (Clupeidae) in Mar Chiquita coastal lagoon: morphological and DNA barcoding identification approaches. Cybium, International Journal of Ichthyology, 36(2), 397–399.
Delpiani, M., González-Castro, M., & Díaz de Astarloa, J. M. (2012). El uso de otolitos y huesos de la cabeza para la identificación de dos especies del género Merluccius, en estudios de predador-presa. Revista de Biología Marina y Oceanografía, 47(2), 351–357. http://www.scielo.cl/scielo.php?pid=S0718-19572012000200018&script=sci_arttext&tlng=pt
González-Castro, M., Ibáñez, A. L., Heras, S., Roldán, M. I., & Cousseau, M. B. (2012). Assessment of lineal versus landmark-based morphometry for discriminating species of Mugilidae (Actinopterygii). Zoological Studies, 51(8), 1515–1528. https://www.researchgate.net/profile/Ana_Ibanez4/publication/260248440_Assessment_of_Lineal_Versus_Landmark-Based_Morphometry_for_Discriminating_Species_of_Mugilidae_Actinopterygii/links/00b7d5374eaa3197dd000000.pdf
Rosso, J. J., Mabragaña, E., González-Castro, M., & Díaz de Astarloa, J. M. (2012). DNA barcoding Neotropical fishes: recent advances from the Pampa Plain, Argentina. Molecular Ecology Resources, 12(6), 999–1011. http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12010/full
Mabragaña, E., Díaz de Astarloa, J. M., Hanner, R., Zhang, J., & González-Castro, M. (2011). DNA Barcoding Identifies Argentine Fishes from Marine and Brackish Waters. PLOS ONE, 6(12), e28655. https://doi.org/10.1371/journal.pone.0028655
Delpiani, S. M., Bruno, D. O., Militelli, M. I., Acuña, F. H., Díaz de Astarloa, J. M., & Gonzalez-Castro, M. (n.d.). Reproductive variables of Hypleurochilus fissicornis (Quoy & Gaimard, 1824) (Pisces: Blenniidae) on rocky intertidal zones in the South-western Atlantic. Journal of the Marine Biological Association of the United Kingdom. https://doi.org/10.1017/S0025315421000230
Dra. Michiels Maria Soledad
Researcher UNMDP
Teaching Assistant
Méndez, E., Michiels, M. S., & López-Mañanes, A. A. (2021). The influence of habitat on metabolic and digestive parameters in an intertidal crab from a SW Atlantic coastal lagoon. Belgian Journal of Zoology, 151, 81–98. https://doi.org/10.26496/bjz.2021.87
Michiels, M. S., Daleo, G. R., & López Mañanes, A. A. (2020). Differential modulation after feeding in different salinities and response to abscisic acid (ABA) and extracellular Ca2+ of aminopeptidase N (APN) activity in the hepatopancreas of the intertidal euryhaline crab Neohelice granulata. Canadian Journal of Zoology, 98(4), 262–268. https://doi.org/10.1139/cjz-2019-0163
Lopez Mañanes, A. A., Asaro, A., Méndez, E., Michiels, M. S., & Pinoni, S. A. (2020). Digestive flexibility in Neohelice granulata from the Mar Chiquita coastal lagoon: Characterization and modulation of key enzymes in hepatopancreas. In E. M. Rodriguez & T. A. Luppi (Eds.), Neohelice granulata, a Model Species for Studies on Crustaceans, Volume II. Cambridge Scholars Publishing. https://ri.conicet.gov.ar/handle/11336/128920
Michiels, M. S., del Valle, J. C., & López Mañanes, A. A. (2017). Trypsin and N-aminopeptidase (APN) activities in the hepatopancreas of an intertidal euryhaline crab: Biochemical characteristics and differential modulation by histamine and salinity. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 204, 228–235.
Del Valle, J. C., Michiels, M. S., Radonic, M., López, A., & López Mañanes, A. A. (2016). Digestive and metabolic profile at the biochemical level of juvenile flounder Paralichthys orbignyanus (Valenciennes, 1839) (Pleuronectiformes: Paralichthyidae). Pan-American Journal of Aquatic Sciences, 11(4), 309–323. http://www.panamjas.org/pdf_artigos/PANAMJAS_11(4)_309-323.pdf
Michiels, M. S., del Valle, J. C., & López Mañanes, A. A. (2015). Lipase activity sensitive to dopamine, glucagon and cyclic AMP in the hepatopancreas of the euryhaline burrowing crab Neohelice granulata (Dana, 1851)(Decapoda, Grapsidae). Crustaceana, 88(1), 51–65. http://booksandjournals.brillonline.com/content/journals/10.1163/15685403-00003399
Michiels, M. S., Del Valle, J. C., & López Mañanes, A. A. (2015). Biochemical characteristics and modulation by external and internal factors of aminopeptidase-N activity in the hepatopancreas of a euryhaline burrowing crab. Journal of Comparative Physiology B, 185(5), 501–510. http://link.springer.com/article/10.1007/s00360-015-0899-3
Pinoni, S. A., Michiels, M. S., & López Mañanes, A. A. (2013). Phenotypic flexibility in response to environmental salinity in the euryhaline crab Neohelice granulata from the mudflat and the saltmarsh of a SW coastal lagoon. Marine Biology, 160(10), 2647–2661.
Michiels, M. S., del Valle, J. C., & López Mañanes, A. A. (2013). Effect of environmental salinity and dopamine injections on key digestive enzymes in hepatopancreas of the euryhaline crab Cyrtograpsus angulatus (Decapoda: Brachyura: Varunidae. Scientia Marina, 77(1), 129–136. http://ri.conicet.gov.ar/handle/11336/2234
Graduate Students    
Dra. Méndez Eugenia
Posdoctoral Fellow – CONICET
Teaching Assistant
Lic. Albanesi Camila Paula
Doctoral Fellow – CONICET